annexin v pe 7aad apoptosis detection kit (Vazyme Biotech Co)

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Vazyme Biotech Co annexin v pe 7aad apoptosis detection kit
Annexin V Pe 7aad Apoptosis Detection Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 290 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/7aad/Annexin+V-PE+7-AAD+Apoptosis+Detection+Kit/pm41932549-68-0-10
Average 96 stars, based on 290 article reviews

annexin v pe 7aad apoptosis detection kit - by Bioz Stars, 2026-10

96/100 stars

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RNA Extraction:

Article Title: Methotrexate-triggered ferroptosis suppresses oral cancer progression by phosphorylated KEAP1-mediated NRF2 degradation to inhibit SLC7A11/GPX4 signaling pathway.
Article Snippet: Some reagents used in this research include Ferrostatin-1 (Fer-1, HY-100579), Z-VAD-FMK (zVAD, HY-16658B), 3-Methyladenine (3-MA, HY-19312), BAY 11-7082 (BAY, HY-13453), Necrosulfonamide (NSA, HY-100573), H2DCFDA (HY-D0940), BODIPY 581/591 C11 (HY-D1301), Cycloheximide (CHX, HY-12320), MG132 (HY-13259), SCH772984 (HY-50846), C16-PAF (HY-108635), Propidium iodide (PI, HY-D0815), ML385 (HY-100523) and TBHQ (HY-100489), which were purchased from MCE. .. RNA isolater total RNA extraction reagent (R401-01), HiScript II Q RT superMix for qPCR (R223-01), ChamQ universal SYBR qPCR master mix (Q711-02), CCK-8 cell counting kit (A311-01), protein A/G magnetic beads (PB101-01), and PE-Annexin V/7AAD apoptosis detection kit (A213-01) were purchased from Vazyme (Nanjing, China). .. Crystal violet staining solution (C0121-100ml), GSH assay kit (S0053), MDA assay kit (S0131S), HRP-labeled goat anti-rabbit IgG(H+L) (A0208), HRP-labeled goat anti-Mouse IgG(H+L) (A0216), Alexa fluor 488-labeled goat anti-Rabbit IgG(H+L) (A0468), AntiFade mounting medium with DAPI (P0131-5ml) were obtained from Beyotime (Shanghai, China).

Article Title: Methotrexate-triggered ferroptosis suppresses oral cancer progression by phosphorylated KEAP1-mediated NRF2 degradation to inhibit SLC7A11/GPX4 signaling pathway
Article Snippet: Some reagents used in this research include Ferrostatin-1 (Fer-1, HY-100579), Z-VAD-FMK (zVAD, HY-16658B), 3-Methyladenine (3-MA, HY-19312), BAY 11–7082 (BAY, HY-13453), Necrosulfonamide (NSA, HY-100573), H2DCFDA (HY-D0940), BODIPY 581/591 C11 (HY-D1301), Cycloheximide (CHX, HY-12320), MG132 (HY-13259), SCH772984 (HY-50846), C16-PAF (HY-108635), Propidium iodide (PI, HY-D0815), ML385 (HY-100523) and TBHQ (HY-100489), which were purchased from MCE. .. RNA isolater total RNA extraction reagent (R401-01), HiScript II Q RT superMix for qPCR (R223-01), ChamQ universal SYBR qPCR master mix (Q711-02), CCK-8 cell counting kit (A311-01), protein A/G magnetic beads (PB101-01), and PE-Annexin V/7AAD apoptosis detection kit (A213-01) were purchased from Vazyme (Nanjing, China). .. Crystal violet staining solution (C0121-100 ml), GSH assay kit (S0053), MDA assay kit (S0131S), HRP-labeled goat anti-rabbit IgG(H + L) (A0208), HRP-labeled goat anti-Mouse IgG(H + L) (A0216), Alexa fluor 488-labeled goat anti-Rabbit IgG(H + L) (A0468), AntiFade mounting medium with DAPI (P0131-5 ml) were obtained from Beyotime (Shanghai, China).

Real-time Polymerase Chain Reaction:

Article Title: Methotrexate-triggered ferroptosis suppresses oral cancer progression by phosphorylated KEAP1-mediated NRF2 degradation to inhibit SLC7A11/GPX4 signaling pathway
Article Snippet: Some reagents used in this research include Ferrostatin-1 (Fer-1, HY-100579), Z-VAD-FMK (zVAD, HY-16658B), 3-Methyladenine (3-MA, HY-19312), BAY 11–7082 (BAY, HY-13453), Necrosulfonamide (NSA, HY-100573), H2DCFDA (HY-D0940), BODIPY 581/591 C11 (HY-D1301), Cycloheximide (CHX, HY-12320), MG132 (HY-13259), SCH772984 (HY-50846), C16-PAF (HY-108635), Propidium iodide (PI, HY-D0815), ML385 (HY-100523) and TBHQ (HY-100489), which were purchased from MCE. .. RNA isolater total RNA extraction reagent (R401-01), HiScript II Q RT superMix for qPCR (R223-01), ChamQ universal SYBR qPCR master mix (Q711-02), CCK-8 cell counting kit (A311-01), protein A/G magnetic beads (PB101-01), and PE-Annexin V/7AAD apoptosis detection kit (A213-01) were purchased from Vazyme (Nanjing, China). .. Crystal violet staining solution (C0121-100 ml), GSH assay kit (S0053), MDA assay kit (S0131S), HRP-labeled goat anti-rabbit IgG(H + L) (A0208), HRP-labeled goat anti-Mouse IgG(H + L) (A0216), Alexa fluor 488-labeled goat anti-Rabbit IgG(H + L) (A0468), AntiFade mounting medium with DAPI (P0131-5 ml) were obtained from Beyotime (Shanghai, China).

CCK-8 Assay:

Article Title: Methotrexate-triggered ferroptosis suppresses oral cancer progression by phosphorylated KEAP1-mediated NRF2 degradation to inhibit SLC7A11/GPX4 signaling pathway
Article Snippet: Some reagents used in this research include Ferrostatin-1 (Fer-1, HY-100579), Z-VAD-FMK (zVAD, HY-16658B), 3-Methyladenine (3-MA, HY-19312), BAY 11–7082 (BAY, HY-13453), Necrosulfonamide (NSA, HY-100573), H2DCFDA (HY-D0940), BODIPY 581/591 C11 (HY-D1301), Cycloheximide (CHX, HY-12320), MG132 (HY-13259), SCH772984 (HY-50846), C16-PAF (HY-108635), Propidium iodide (PI, HY-D0815), ML385 (HY-100523) and TBHQ (HY-100489), which were purchased from MCE. .. RNA isolater total RNA extraction reagent (R401-01), HiScript II Q RT superMix for qPCR (R223-01), ChamQ universal SYBR qPCR master mix (Q711-02), CCK-8 cell counting kit (A311-01), protein A/G magnetic beads (PB101-01), and PE-Annexin V/7AAD apoptosis detection kit (A213-01) were purchased from Vazyme (Nanjing, China). .. Crystal violet staining solution (C0121-100 ml), GSH assay kit (S0053), MDA assay kit (S0131S), HRP-labeled goat anti-rabbit IgG(H + L) (A0208), HRP-labeled goat anti-Mouse IgG(H + L) (A0216), Alexa fluor 488-labeled goat anti-Rabbit IgG(H + L) (A0468), AntiFade mounting medium with DAPI (P0131-5 ml) were obtained from Beyotime (Shanghai, China).

Cell Counting:

Article Title: Methotrexate-triggered ferroptosis suppresses oral cancer progression by phosphorylated KEAP1-mediated NRF2 degradation to inhibit SLC7A11/GPX4 signaling pathway
Article Snippet: Some reagents used in this research include Ferrostatin-1 (Fer-1, HY-100579), Z-VAD-FMK (zVAD, HY-16658B), 3-Methyladenine (3-MA, HY-19312), BAY 11–7082 (BAY, HY-13453), Necrosulfonamide (NSA, HY-100573), H2DCFDA (HY-D0940), BODIPY 581/591 C11 (HY-D1301), Cycloheximide (CHX, HY-12320), MG132 (HY-13259), SCH772984 (HY-50846), C16-PAF (HY-108635), Propidium iodide (PI, HY-D0815), ML385 (HY-100523) and TBHQ (HY-100489), which were purchased from MCE. .. RNA isolater total RNA extraction reagent (R401-01), HiScript II Q RT superMix for qPCR (R223-01), ChamQ universal SYBR qPCR master mix (Q711-02), CCK-8 cell counting kit (A311-01), protein A/G magnetic beads (PB101-01), and PE-Annexin V/7AAD apoptosis detection kit (A213-01) were purchased from Vazyme (Nanjing, China). .. Crystal violet staining solution (C0121-100 ml), GSH assay kit (S0053), MDA assay kit (S0131S), HRP-labeled goat anti-rabbit IgG(H + L) (A0208), HRP-labeled goat anti-Mouse IgG(H + L) (A0216), Alexa fluor 488-labeled goat anti-Rabbit IgG(H + L) (A0468), AntiFade mounting medium with DAPI (P0131-5 ml) were obtained from Beyotime (Shanghai, China).

Magnetic Beads:

Article Title: Methotrexate-triggered ferroptosis suppresses oral cancer progression by phosphorylated KEAP1-mediated NRF2 degradation to inhibit SLC7A11/GPX4 signaling pathway
Article Snippet: Some reagents used in this research include Ferrostatin-1 (Fer-1, HY-100579), Z-VAD-FMK (zVAD, HY-16658B), 3-Methyladenine (3-MA, HY-19312), BAY 11–7082 (BAY, HY-13453), Necrosulfonamide (NSA, HY-100573), H2DCFDA (HY-D0940), BODIPY 581/591 C11 (HY-D1301), Cycloheximide (CHX, HY-12320), MG132 (HY-13259), SCH772984 (HY-50846), C16-PAF (HY-108635), Propidium iodide (PI, HY-D0815), ML385 (HY-100523) and TBHQ (HY-100489), which were purchased from MCE. .. RNA isolater total RNA extraction reagent (R401-01), HiScript II Q RT superMix for qPCR (R223-01), ChamQ universal SYBR qPCR master mix (Q711-02), CCK-8 cell counting kit (A311-01), protein A/G magnetic beads (PB101-01), and PE-Annexin V/7AAD apoptosis detection kit (A213-01) were purchased from Vazyme (Nanjing, China). .. Crystal violet staining solution (C0121-100 ml), GSH assay kit (S0053), MDA assay kit (S0131S), HRP-labeled goat anti-rabbit IgG(H + L) (A0208), HRP-labeled goat anti-Mouse IgG(H + L) (A0216), Alexa fluor 488-labeled goat anti-Rabbit IgG(H + L) (A0468), AntiFade mounting medium with DAPI (P0131-5 ml) were obtained from Beyotime (Shanghai, China).

TUNEL Assay:

Article Title: Human Umbilical Cord Blood Plasma‐Derived Exosomal miR‐410‐3p Alleviates Liver Injury by Regulating the Mitochondria‐Mediated Antiapoptotic Signaling
Article Snippet: ROS burst and scavenging in cells was assessed by DCFH-DA (beyotime, China) probe and quantified by fluorescent microscope. .. Cell apoptosis was investigated by TUNEL staining (Beyotime, China) or analyzed by Annexin V-PE/7AAD Apoptosis Detection Kit (Vazyme, China). .. Mitochondrial injury and dysfunction regarding as mitochondrial permeability transition pore (mPTP), mitochondrial membrane potential (MMP) after treatments were detected by a Calcein AM (beyotime, China) and AnnexinV/Mito-tracker detection kit (beyotime, China), respectively.

Staining:

Article Title: BCAT2 binding to PCBP1 regulates the PI3K/AKT signaling pathway to inhibit autophagy-related apoptosis and ferroptosis in prostate cancer.
Article Snippet: .. Floating cells in the wells were harvested, while the remaining cells were digested, harvested with trypsin (Gibco), resuspended, and stained with binding buffer in accordance with the instructions of the Annexin V-PE/7AAD Apoptosis Detection Kit (Vazyme, Nanjing, China). .. The proportion of apoptotic cells was determined by flow cytometry (BD FACSCanto II; BD Biosciences, Franklin, NJ, USA).

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